2．   CD三z哆兽．e矿C^P玎l厶t，)，口厅d C^em如Ⅱf E，lgi，}eeri，皤，尸i7皤di，秽危Dn C胁眈邝i纱，Pi，wdi，拶危Ⅱn    467000，c^i厅口)

Abstract：Three dimensional nuorescence spectmm  combined with two kinds of 8econd—order   calibration  algo•
rithms  with physical separation  panially  substituted  by mathematical separation was deVeloped fbr simultaneous quantitative dete咖ination of Fleroxacin(FLE)，Penoxacin(PEF) and Enoxacin(ENO)  in milk． The pro— posed method can perfbm simultaneous quantitative dete珊ination of interesting analytes  in the presence of milk inted’erence without separating the three kinds of quinolone8，and the operation proces8 is simple and has
high  sensitivity and selectivity and satisfactory prediction  results．AATLD(Altemating asymmetric trilinear de- comp08ition)  analysis  result showed that the average recoverie8 of Fleroxacin(FLE)，Penoxacin(PEF) and enoxacin(ENO)are(97．87±4．91)％，(97．55±5．45)％and(102．13±5．759)％respectively．
Key words：neroxacin；penoxacin；enoxacin；three   dimensional fluorescence；second—order calibration

 

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